Dynamic light scattering (DLS) is an analytical technique used to measure the size distribution and concentration of particles in a sample. This technique works by measuring the angle of light scattered off a particle in solution.
The intensity of the scattered light is proportional to the particle size, and the angle of the scattered light is proportional to the particle’s diffusion coefficient. DLS is a non-invasive, real-time technique that can be used to study a variety of particle systems. It can measure particles from nanometers to millimeters in size and can be used to analyze samples in the presence of other components such as proteins, micelles, and colloids.
In a typical DLS system, light from a laser or LED is focused onto the sample solution. The sample is usually contained in an optical cell, which is placed in an optical module. The scattered light is detected by a photodetector, which is connected to a computer. The computer then processes the information and displays the results in the form of a graph. The graph shows the intensity of the scattered light as a function of the particle size.
DLS is widely used in many fields, including chemistry, material science, biophysics, and nanotechnology. It can be used to study the stability of proteins, the size distribution of nanoparticles, and the structure of colloids and emulsions.
It is also used to monitor the aggregation of particles in solution and to analyze the properties of surfactants and dispersants. The technique is fast, reliable, and easy to use, making it a popular choice for many research and industrial applications.
The Global Dynamic light scattering (DLS) system market accounted for $XX Billion in 2022 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2023 to 2030.
Microtrac has been developing optical systems based on Dynamic Light Scattering and is a pioneer in particle analysis technology. Microtrac offers the best accuracy, resolution, and sensitivity by combining the advantages of a short route length with reference beats and 180° backscatter by focussing the laser probe at the material contact.
A method known as “beating” is used in all dynamic light scattering experiments to remove the high optical frequency from the scattered light and leave behind the lower frequencies caused by particle motion that are necessary for size analysis. The probe gathers 180° backscattered light mixed with incident light in order to implement Microtrac’s heterodyne detection technology.
The interface’s design allows light to reflect and blend with scattered light that has been gathered. It is possible to beat references thanks to reflected light. Because the reflected component is highly intense, the entire optical signal is magnified. Optimal optical signal quality is achieved at the lowest concentrations, resulting in precise measurements.T
he Laser is focused at the interface between the particle suspension and the Microtrac probe in dynamic light scattering analyzers. A 180° backscattered light and the contacted particles cause the suspension to scatter light once light enters.
It is redirected to the photodetector mixed with incident light. Maximum dispersed light collection and minimum overall path length are achieved. The highest particle concentrations are measured accurately as a result.
Since it provides details regarding the motion of the particles, the temporal fluctuation of the dispersed light signal is significant in this context. Because the light-spreading particles are moving in relation to one another, the interferences within the total amount of dispersed light are continually changing, which is what causes the variations.
As a result, there are small frequency shifts in the light that the particles scatter due to their position or velocity changing over time. Motion generates a variety of frequency shifts when measured over time. By comparing these shift frequencies with a coherent optical reference, they can be found. The shift frequencies in dynamic light scattering are easily measurable and fall between 1 Hz and 100 KHz.
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