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In order to investigate the relationship between neural activity and animal behaviours, fibre photometry system, an optical imaging technique, allows researchers to monitor the activity of huge neuronal populations in freely moving animals for an extended period of time.
The idea behind fibre photometry is to use fibre optics and time-correlated single-photon counting (TCSPC) to measure the light emitted by fluorescent molecules.
The low-cost implanted cannula used by the fibre photometry device eliminates the need for an overhead miniscope. The lengthy studies on freely acting animals are made possible by easy animal surgery, light implants, and lower data sets.
The fibre photometry device is combined with GCamP, one of the genetically encoded calcium indicators (GECIs), to record neuronal events because of its high signal-to-noise ratio and versatility in behavioural paradigms.
Despite producing the highest level of temporal precision, electrophysiological performs poorly in detecting cell-type-specific population neuronal activity of the deep brain structures in behaving animals.
The Global Fiber Photometry System Market accounted for $XX Billion in 2022 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2023 to 2030.
Multi-Wavelength Multi-Fiber Photometry System by Plexon. The traditional single-wavelength photometry system’s technology is expanded upon in the new multi-wavelength photometry system, which also includes additional features based on user feedback.
This camera-based system has two optical sensors that can concurrently detect green and red fluorescence, along with three integrated LEDs for excitation. The user can record from various brain areas or various animals thanks to a branching patch wire.
The device interleaves the 465 nm and 410 nm LEDS, and the camera that detects green fluorescence independently collects light for each of these wavelengths. This enables the user to recognise the calcium-dependent fluorescence (isobestic control).
With two excitation light sources, RWD’s R810 Dual Color Multichannel Fiber Photometry System is available (410nm and 470nm).
To ensure the collection of actual fluorescence data, the background noise signal can be reflected using the 410 nm wavelength.
Three excitation light sources are present in the R820 Tri-Color Multichannel Fiber Photometry System (410nm, 470nm and 560nm).
The system can record the signal from a neurotransmitter probe or a green fluorescence indication like GCaMP and dLight, as well as a red fluorescence indicator like RCaMP and jrGECO1a.
A strong new fibre photometry system can record red and green fluorescence signals, including GCaMP, RCaMP, dLight, and jrGECO1a, while a special 410nm LED is utilised to capture control signals and exclude noise. The system also features an integrated lightweight body and richer software.
