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A mercury or xenon lamp is used in a fluorescence microscope to produce ultraviolet light. When light enters the microscope, it strikes a dichroic mirror, which allows some wavelengths to pass through while reflecting others. The ultraviolet light is reflected by the dichroic mirror up to the specimen.
Nucleic acid stains that attach to the minor groove of DNA and mark cell nuclei include DAPI, Hoechst, and DRAQ5 and DRAQ7, which are all best stimulated by red light and UV wavelength light, respectively.
The imaging of specific features of tiny objects, such as microorganisms, is frequently done using fluorescent microscopy. At smaller scales, it is also utilized to visually improve 3-D features.
This can be done by adding fluorescent tags to antibodies that bind to certain characteristics or by less-targeted labeling.
The global fluorescence microscope market accounted for $XX Billion in 2023 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2024 to 2030.
Live-cell fluorescence microscopy is a potent technique with several uses. The low-maintenance CytoSMART Omni FL overcomes the limitations of other imaging platforms and enables plug-and-play experimentation right out of the box.
It does this by having an innovative design that operates efficiently in a cell culture incubator, universal compatibility with any transparent culture vessel, AI-driven analysis with user-friendly data storage solutions, and universal compatibility with transparent culture vessels.
Examining the health and viability of individual cells, colony formation and wound healing, transfection effectiveness, and complicated culture models like co-cultures and 3D organoids are all examples of applications for live-cell fluorescence imaging.
The Lux3 FL Duo Kit and the Multi Lux3 FL, two new fluorescence live-cell imaging devices, have been introduced by Cytosmart Technologies. A portable and reasonably priced imaging device with two fluorescence microscopes for side-by-side examination of cell cultures is the CytoSmart Lux3 FL Duo Kit.
The CytoSmart Multi Lux3 FL fluorescence live-cell imaging system is intended for large research teams and long-term comparative investigations. It consists of four small Lux3 FL devices with two fluorescent (green and red) and one brightfield channel.
Both CytoSmart fluorescent live-cell imaging platforms work in a conventional CO2 incubator or hypoxic chamber, enabling users to examine cells in the culture environment of their choice.
