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Alanine, often known as -alanine, is an amino acid that is employed in the biosynthesis of proteins. Its symbol is Ala or A.
The centre carbon atom, which also carries a methyl group side chain, is connected to an amine group and a carboxylic acid group, respectively. As a nonpolar, aliphatic -amino acid, it is referred to by its IUPAC systematic name, 2-aminopropanoic acid.
L-alanine is one of the smallest chiral molecules and has a long history of application in the veterinary, pharmaceutical, and food industries.
For instance, it is utilised in clinical medicine along with other L-amino acids as a pre- and postoperative nutrition therapy and in the food sector as a sweetener due to its sweet flavour. Due to advancements in science, technology, and economics, more uses for L-alanine are being gradually discovered.
The amino acid alanine is necessary for the synthesis of proteins. Vitamin B-6 and tryptophan are broken down using it. It provides the central nervous system and muscles with energy. It helps the body use glucose and boosts the immune system.
In commercial manufacturing, immobilised cells or cell suspensions of microorganisms with a L-aspartic-decarboxylase were used as the biocatalyst to create L-alanine by enzymatic decarboxylation of L-aspartic acid. The yield of L-alanine and the high efficiency of this process have both been demonstrated.
The Global L-Alanine market accounted for $XX Billion in 2022 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2023 to 2030.
Metabolic engineering of microorganisms for L-alanine production.In the culinary, pharmaceutical, and chemical industries, l-alanine is widely employed. L-aspartic acid derived from petroleum has been used as the substrate for the majority of the enzymatic process used in industrial manufacture of L-alanine.
An alternate method is to produce L-alanine from renewable biomass via microbial fermentation. Using aminotransferase or L-alanine dehydrogenase, several bacteria may naturally generate L-alanine. However, because of their poor yields and productivity, generation of L-alanine using native strains has been constrained. metabolic engineering of microbes for the production of L-alanine.
The anaerobic fermentation process for L-alanine has a number of benefits, most notably a high L-alanine yield, and it was the first to achieve commercialization. The first amino acid that could be generated commercially using anaerobic fermentation is L-alanine.
