A super-resolution image is created using stochastic optical reconstruction microscopy (STORM), which combines the highly accurate localization data of individual fluorophores in three dimensions and numerous colours.
The activated state of a photoswitchable molecule is the basic idea behind stochastic optical reconstruction microscopy (STORM) and associated methodology.
Before it enters a dark state or is rendered inactive by photobleaching, it must cause the subsequent emission of enough photons to allow precise localization.
The Global Stochastic optical reconstruction microscopy market accounted for $XX Billion in 2022 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2023 to 2030.
For STORM Super-Resolution Microscopy, Biotium has released optimised antibody labelling kits. Biotium offers Mix-n-Stain STORM CF Dye Antibody Labelling Kits with these dyes, which have remarkable brightness and photochemical properties perfect for STORM imaging.
The packages are also developed to produce low labelling levels that are ideal for single molecule localization microscopy methods like STORM.
The fluorophore and antibody conjugate’s characteristics have a significant impact on the clarity of STORM images.
For STORM, in particular, bright fluorophores with strong photo-switching characteristics are perfect because they can switch on and off quickly even at low labelling levels.
To make antibody conjugates with little to no STORM tagging, however, there isn’t a practical way to do it.
Additionally, there aren’t many choices for green-excited fluorescent probes for multi-color STORM, whereas there are a variety of red-excited dyes that are suitable for STORM.
This problem has been met by Biotium with the introduction of Mix-n-Stain STORM CF Dye Antibody Labelling Kits, which provide a streamlined method for creating antibody conjugates ideal for STORM imaging in a range of colours.
Eight CF Dyes that have been validated by STORM and have emission ranging from visible red to near-IR are offered in Mix-n-Stain STORM CF Dye Antibody Labelling Kits.
Novel green-excited CF dyes for multi-color STORM, CF 583R and CF 597R, were just released in association with a UC Berkeley researcher.
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