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Last Updated: Apr 25, 2025 | Study Period: 2024-2030
A straightforward fluorescence-based assay for checking the viability of mammalian cells is described. It is based on the observation of cell respiration using a phosphorescent, water-soluble oxygen probe that adapts its emission intensity and lifetime in response to changes in the concentration of dissolved oxygen.
Low quantities of the probe (0.3 microM to 0.5 nM) were introduced to each sample, which was a cell culture in a well of a typical 96-well plate. Mineral oil was applied to the top of each sample to start the analysis of oxygen consumption. This was followed by the observation of the phosphorescent signal on a prompt or time-resolved fluorescence plate reader.
On the basis of kinetic variations in the phosphorescence (initial slopes), rates of oxygen uptake might be calculated.
The Global Fluorescence-Based Viability Assays market accounted for $XX Billion in 2023 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2024 to 2030.
Fluorescence cell-based viability assays as a new tool for high-throughput screening, diagnostic assays. Similar to e-noses and e-tongues, which integrate semi-specific sensors and multivariate data analysis for monitoring biochemical processes, this very sensitive optical assay works in a similar manner.
An environmental-sensitive fluorescent dye mixture and human skin cells used in the optical assay produce fluorescence spectrum patterns specific to different physico-chemical and physiological situations. The optical signal is analysed using chemometric methods to provide qualitative details about the analytical properties of the substances.
This comprehensive technique has been effectively used to determine whether or not specific chemical compounds are irritating to human skin (with sensitivity of 93% and specificity of 97%).
Sl no | Topic |
1 | Market Segmentation |
2 | Scope of the report |
3 | Abbreviations |
4 | Research Methodology |
5 | Executive Summary |
6 | Introduction |
7 | Insights from Industry stakeholders |
8 | Cost breakdown of Product by sub-components and average profit margin |
9 | Disruptive innovation in the Industry |
10 | Technology trends in the Industry |
11 | Consumer trends in the industry |
12 | Recent Production Milestones |
13 | Component Manufacturing in US, EU and China |
14 | COVID-19 impact on overall market |
15 | COVID-19 impact on Production of components |
16 | COVID-19 impact on Point of sale |
17 | Market Segmentation, Dynamics and Forecast by Geography, 2024-2030 |
18 | Market Segmentation, Dynamics and Forecast by Product Type, 2024-2030 |
19 | Market Segmentation, Dynamics and Forecast by Application, 2024-2030 |
20 | Market Segmentation, Dynamics and Forecast by End use, 2024-2030 |
21 | Product installation rate by OEM, 2023 |
22 | Incline/Decline in Average B-2-B selling price in past 5 years |
23 | Competition from substitute products |
24 | Gross margin and average profitability of suppliers |
25 | New product development in past 12 months |
26 | M&A in past 12 months |
27 | Growth strategy of leading players |
28 | Market share of vendors, 2022 |
29 | Company Profiles |
30 | Unmet needs and opportunity for new suppliers |
31 | Conclusion |
32 | Appendix |