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A straightforward fluorescence-based assay for checking the viability of mammalian cells is described. It is based on the observation of cell respiration using a phosphorescent, water-soluble oxygen probe that adapts its emission intensity and lifetime in response to changes in the concentration of dissolved oxygen.
Low quantities of the probe (0.3 microM to 0.5 nM) were introduced to each sample, which was a cell culture in a well of a typical 96-well plate. Mineral oil was applied to the top of each sample to start the analysis of oxygen consumption. This was followed by the observation of the phosphorescent signal on a prompt or time-resolved fluorescence plate reader.
On the basis of kinetic variations in the phosphorescence (initial slopes), rates of oxygen uptake might be calculated.
The Global Fluorescence-Based Viability Assays market accounted for $XX Billion in 2023 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2024 to 2030.
Fluorescence cell-based viability assays as a new tool for high-throughput screening, diagnostic assays. Similar to e-noses and e-tongues, which integrate semi-specific sensors and multivariate data analysis for monitoring biochemical processes, this very sensitive optical assay works in a similar manner.
An environmental-sensitive fluorescent dye mixture and human skin cells used in the optical assay produce fluorescence spectrum patterns specific to different physico-chemical and physiological situations. The optical signal is analysed using chemometric methods to provide qualitative details about the analytical properties of the substances.
This comprehensive technique has been effectively used to determine whether or not specific chemical compounds are irritating to human skin (with sensitivity of 93% and specificity of 97%).
