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Fluorescence imaging works by shining a specific wavelength of light, ideally close to the peak of the fluorophore excitation spectrum, on fluorescently tagged proteins or other intracellular molecules and then detecting the light that is released at a longer wavelength. How much excitation light is actually required to produce a good image is a crucial question.
Widefield epifluorescence illumination can be calculated similarly, and depending on the light source, the results are similar. The typical irradiance levels for laser scanning confocal microscopes can be several orders of magnitude greater since they use a focussed beam to illuminate a relatively small area at a tim
Modern cell biology now includes fluorescence microscopy of living cells as a necessary component. A variety of instruments are available to study practically any biological function under the microscope thanks to fluorescent protein tags, live cell dyes, and other techniques to fluorescently mark proteins of interest.
To reduce photodamage while maintaining a usable signal-to-noise ratio and to provide a suitable environment for cells or tissues to imitate physiological cell dynamics are the two key experimental problems in gathering meaningful live cell imaging data.
Spinning disk confocal microscopes are preferable for live cell imaging due to the difference in specimen irradiance between them and laser scanning confocal microscopes.
As long as the majority of fluorescent molecules in a population are not in the excited state, fluorescence emission is linearly linked to the intensity of the excitation light.
However, at higher photon flux rates, which can be achieved very readily with laser scanning confocal microscopes, a significant fraction of fluorophores occupy the excited state and are unable to absorb any more photons.
Additional excitation light will only result in sub-proportional increases in fluorescence signal during ground-state depletion, but it will still cause photodamage.
Ground-state depletion is not attained even with excitation lasers with hundreds of mW power output in spinning disk confocal microscopes because the excitation laser light is dispersed among thousands of pinholes that scan across the object quickly.
The Global fluorescence live cell imaging market accounted for $XX Billion in 2022 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2023 to 2030.
Axion Bio subsidiary CytoSMART Technologies has introduced the CytoSMART Omni FL, a next-generation live-cell imaging analysis system that for the first time adds red and green fluorescence channels to its renowned CytoSMART Omni product range.
The development is a reflection of the company’s continuous dedication to offering each cell biology lab high-quality, affordable live-cell imaging, and it provides a cutting-edge platform for researchers in stem cell biology, immuno-oncology, virology, toxicology, neurology, and other domains.
This invention is the next significant step in our ongoing product line innovation plan, which is intended to hasten scientific research and medicine development.
In biological, live-cell fluorescence microscopy is a potent technique with several uses. Imaging offers a crucial window into the physiology and function of cells over time and makes it possible to produce high-quality time-lapse recordings to follow intricate cellular processes. Fluorescent tags, dyes, and other techniques are used to label and study molecules of interest.
The low-maintenance CytoSMART Omni FL addresses the drawbacks of previous intelligent devices with its novel design that functions effectively in a cell culture incubator, universal compatibility with any transparent culture vessel, and AI-driven analysis with user-friendly data storage solutions.
