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Fluorescent Western blotting, often known as fluorescence Western blotting or simply “fluorescent Western,” is a variant of the classic Western blotting technique that is used to detect and quantify particular proteins in a biological sample. While classic Western blotting uses enzymatic or chemiluminescent detection methods, fluorescent Western blotting visualizes target proteins using fluorescent labeling. Gel electrophoresis is used to sort proteins based on their molecular weight.
The separated proteins are then transported to a membrane, which is commonly made of nitrocellulose or PVDF. To excite the fluorescent dye bound to the secondary antibody, the membrane is exposed to light of the proper wavelength. A fluorescence imaging system detects and records light emitted by the dye at a longer wavelength.
Numerous fluorescent dyes can be employed at the same time to mark distinct target proteins, allowing numerous proteins to be detected in the same sample. Fluorescence detection provides a greater dynamic range than other detection technologies, enabling for the detection of both high and low abundance proteins.
Many current imaging systems can detect fluorescence and chemiluminescence, allowing for greater experiment design freedom. Fluorescence signals are often more stable over time than chemiluminescent signals, which fade rather quickly. Fluorescent Western blotting is a strong technique that has grown in popularity due to its capacity to give quantitative and multiplexed protein analysis, making it a vital tool in molecular biology, biochemistry, and biomedical research.
The Global Fluorescent Western Blotting market accounted for $XX Billion in 2023 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2024 to 2030.
Western Bright MCF fluorescent visible and near infrared (IR) Western blotting kits enable the simultaneous detection of two proteins, enhancing the quality and quantity of information obtained from a single blot. Using dyes with various spectral qualities (excitation and emission), you can see them in multiple channels on a digital imager at the same time. Simultaneous detection with distinct antibodies, often known as multiplexing, is useful for some sorts of studies.
As an example, test a loading control in conjunction with a protein of interest. Furthermore, the Western Bright methodology saves time and money by eliminating the need to strip and re-probe a blot, eliminating the requirement for disposable film, and allowing the blot to be imaged immediately without drying.
