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Last Updated: Apr 25, 2025 | Study Period: 2024-2030
Protein transfer, which includes moving proteins electrophoretically separated in a gel to a solid support matrix, is an essential step in western blot analysis.The two most popular means of protein transfer are as follows:
Electrophoretic transfer is the process by which proteins that have been separated into gels (for instance, following polyacrylamide gel electrophoresis, or PAGE) are transferred onto membranes.
Because haemoglobin transports oxygen from the lungs to other parts of the human body, it is referred to as a transport protein or carrier protein.The protein mixture is separated, and then it is moved to a membrane.
Proteins move out of the gel and onto the membrane during the transfer utilising an electric field that is perpendicular to the gel's surface. In a sandwich configuration, the membrane is positioned between the gel surface and the positive electrode.
It is necessary to position the membrane in this way for the negatively charged proteins to go from the gel to the membrane. Electrophoretic transfer is a sort of transfer that can be carried out in either wet or semi-dry conditions.
Larger proteins are favoured in moist settings since they are less prone to dry out the gel and are generally more trustworthy.Transferring proteins from the polyacrylamide gel to the nitrocellulose or polyvinylidene difluoride (PVDF) membrane after electrophoresis is one of the crucial phases in the western blot workflow so that particular proteins can be recognised using immune-detection methods.
The most widely used method for IEF, gel electrophoresis, reduces convection and adds a second gel-sieving effect to separate proteins according to size. However, it has a number of drawbacks, including a long analysis time, low resolution, and challenging detection.
The Global protein transfer system accounted for $XX Billion in 2022 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2024 to 2030.
Wet, semi-dry, and dry blotting techniques have electrophoretic transfer systems invented by thermo fisher.Blotting surface together with Transfer buffer volume of 220 mL per blot module; Mini Blot Module with Capacity of 1 Mini Gel per Blot Module; 1-2 Blot Modules per Tank.
One of the most important processes in the western blot workflow is the transfer of proteins from the polyacrylamide gel after electrophoresis to the nitrocellulose or polyvinylidene difluoride (PVDF) membrane so that particular proteins can be recognised using immune-detection methods.
Thermo Fisher Scientific Inc.'s iBlot 3 Western Blot Transfer System combines tried-and-true iBlot technology with a cutting-edge, brand-new, sturdy design to give great performance and simplicity.
With an integrated power supply and pre-packaged, ready-to-use transfer stacks, the iBlot 3 device is a genuine plug-and-play, self-contained device. Compared to the far more laborious and physically demanding methods needed for wet-tank transfer, setup, disassembly, and cleanup just take a few minutes.
Sl no | Topic |
1 | Market Segmentation |
2 | Scope of the report |
3 | Abbreviations |
4 | Research Methodology |
5 | Executive Summary |
6 | Introduction |
7 | Insights from Industry stakeholders |
8 | Cost breakdown of Product by sub-components and average profit margin |
9 | Disruptive innovation in the Industry |
10 | Technology trends in the Industry |
11 | Consumer trends in the industry |
12 | Recent Production Milestones |
13 | Component Manufacturing in US, EU and China |
14 | COVID-19 impact on overall market |
15 | COVID-19 impact on Production of components |
16 | COVID-19 impact on Point of sale |
17 | Market Segmentation, Dynamics and Forecast by Geography, 2024-2030 |
18 | Market Segmentation, Dynamics and Forecast by Product Type, 2024-2030 |
19 | Market Segmentation, Dynamics and Forecast by Application, 2024-2030 |
20 | Market Segmentation, Dynamics and Forecast by End use, 2024-2030 |
21 | Product installation rate by OEM, 2022 |
22 | Incline/Decline in Average B-2-B selling price in past 5 years |
23 | Competition from substitute products |
24 | Gross margin and average profitability of suppliers |
25 | New product development in past 12 months |
26 | M&A in past 12 months |
27 | Growth strategy of leading players |
28 | Market share of vendors, 2022 |
29 | Company Profiles |
30 | Unmet needs and opportunity for new suppliers |
31 | Conclusion |
32 | Appendix |