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Transmission electron microscopy (TEM) sample preparation is the primary application of ultramicrotomy. It enables the visualization and analysis of samples’ internal fine structures at the nanometer scale. It quickly and cleanly produces ultrathin sections of samples.
The size and uniformity of the electron-transparent area within the sections, as well as the speed at which the sections are produced, are two significant advantages of ultramicrotomy.
The ultramicrotomy technique can be used on a variety of samples, such as biological specimens and industrial materials like rubber and plastic polymers and ductile, hard, or brittle materials like metals and ceramics.
Ultramicrotomy has advantages in terms of speed and cleanliness over other methods for preparing thin sections of these samples, such as focused ion beam (FIB) milling, ion etching, tripod polishing, and electrochemical processing.
For cellular and protein structure analysis, array tomography (AT) is a high-resolution, three-dimensional image reconstruction technique.
It is carried out by imaging ultrathin, resin-embedded serial sections in ordered arrays with scanning electron microscopy (SEM) or light microscopy (LM) techniques. Quantitative, volumetric structural analysis and visualization of protein and cellular structures are made possible by AT.
Contrary to conventional confocal microscopy, it has higher lateral and spatial resolution. The examination of biospecimens can also be partially automated to achieve a higher throughput.
The Global Ultramicrotome Market accounted for $XX Billion in 2023 and is anticipated to reach $XX Billion by 2030, registering a CAGR of XX% from 2024 to 2030.
Transmission electron microscope (TEM) observation of ultra-thin sections has been extensively utilized for the morphological study of polymers. Their function has greatly improved as a result of this method. On the other hand, this method typically yields a range that is only about 100 nm thick and only a few hundred m square.
Imaging with resolution comparable to that of a TEM is now possible thanks to advancements in scanning electron microscopes (SEMs) and their detectors. Subsequently, SEM constant crosssectional perception technique for procuring consistent tomographic pictures with SEM and
reproducing three aspects is broadly utilized in the natural review. Although it includes a number of method, array tomography is a simple technique that only requires an ultramicrotome and a scanning electron microscope (SEM) to observe continuous ultrathin sections prepared with an ultramicrotome.
However, the fact that sophisticated methods are required for the preparation and collection of serial sections has been a drawback. Leica Microsystems introduced a new ultramicrotome to address this issue, which uses a novel collection method to automatically produce and collect high-quality serial sections at a high throughput.
